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KMID : 0880220130510040423
Journal of Microbiology
2013 Volume.51 No. 4 p.423 ~ p.429
Multiple gene genealogical analyses of a nematophagous fungus Paecilomyces lilacinus from China
Juan Li

Heng Li
Xiaoxu Bi
Ke-Qin Zhang
Abstract
Paecilomyces lilacinus is a geographically widespread nematophagous fungus and a promising biological control agent against plant parasitic nematodes. However, relatively little is known about its patterns of genetic variation through its broad geographic and ecological contexts. In this study, we analyzed the genetic variation of 2 virulence-associated genes (PLS and PLC) and 4 housekeeping gene fragments (ITS, RPB1, RPB2, and ¥â-tubulin) among 80 P. lilacinus specimens collected from 7 locations in China. Various degrees of polymorphism and haplotype diversity were observed among the six gene fragments. However, no genetic differentiation was observed among the geographic populations, consistent with extensive gene flow among these geographic populations of P. lilacinus in China. Our analysis also suggested that clonal reproduction was the predominant mode of reproduction in natural populations of P. lilacinus.
KEYWORD
genetic diversity, biological control, haplotype, clonal reproduction
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